Indox Consulting works with medical professionals who practice specifically in the field of DNA analysis and are capable of performing independent DNA testing and analysis of previously performed DNA tests. Currently, there are two main types of forensic DNA testing. They are RFLP analysis and PCR-based analysis.
Generally, RFLP analysis requires larger amounts of DNA and the DNA must be undegraded. Crime-scene evidence that is old or that is present in small amounts is often unsuitable for RFLP testing. Warm moist conditions may accelerate DNA degradation rendering it unsuitable for RFLP in a relatively short period of time.
PCR testing often requires less DNA than RFLP testing and the DNA may be partially degraded, more so than is the case with RFLP. However, PCR still has sample size and degradation limitations. PCR tests are extremely sensitive to contaminating DNA at the crime scene and within the test laboratory. During PCR, contaminants may be amplified up to a billion times their original concentration. Contamination can influence PCR results, particularly in the absence of proper handling techniques and proper controls for contamination.
For an excellent introduction to the field of DNA analysis for laymen,
DNA Primer.